梁爱惠;刘高伞;蒋治良;

• 1:广西师范大学环境与资源学院
• 2:珍稀濒危动植物生态与环境保护省部共建教育部重点实验室

摘要(Abstract)：

在pH8.0的Tris-HCl缓冲溶液及32mg/L PEG20000的存在下,碱性磷酸酯酶(ALP)催化底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)水解生成不溶性的蓝色BCIP二聚体微粒,该微粒在710nm波长处产生一个共振散射峰。在选定条件下,随着ALP活性增大,710nm波长处的共振散射峰强度线性增大,碱性磷酸酯酶浓度在0.078～1.25U/L与共振散射强度增大值ΔI呈良好线性关系,其回归方程为:ΔI=512.6C+17.8,检出限为9.6×10-3U/L。该法用于合成样品中碱性磷酸酯酶的测定,结果满意。

关键词(KeyWords)： 5-溴-4-氯-3-吲哚基磷酸盐;碱性磷酸酯酶;共振散射光谱法

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(21165005,21075023);; 广西研究生科研创新项目资助(2011106020830M62)

作者(Authors): 梁爱惠;刘高伞;蒋治良;

DOI: 10.16088/j.issn.1001-6600.2012.02.011

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