卵形鲳鲹Myostatin基因克隆及其在胚胎发育中的表达分析Molecular Cloning of the Myostatin Gene and Its Expression During Embryo Development of Trachinotus ovatus
罗洪林;冯鹏霏;余艳玲;肖蕊;潘传燕;宋漫玲;张永德;
摘要(Abstract):
为研究肌生长抑制素(myostatin, MSTN)在卵形鲳鲹胚胎发育过程中的表达,对卵形鲳鲹MSTN基因CDS区进行克隆与分析,并对其14个胚胎发育阶段的表达量进行研究。结果显示:克隆获得MSTN基因1 322 bp序列。该序列编码376个氨基酸,相对分子质量为42 694.85 u,理论等电点pI为5.52,为带负电的蛋白。MSTN蛋白质不存在跨膜结构域,亚细胞定位主要于细胞外部,可能属于分泌蛋白质,易于表达和纯化。MSTN蛋白质无信号肽序列,预测含有17个磷酸化位点。对24种鱼类MSTN基因序列的同源分析发现:卵形鲳鲹MSTN与布氏鲳鲹同源性最高,与黄尾鰤同源性次之;3种鱼类均属鲹科Carangidae,而鲹科鱼类与锯盖鱼科Centropomidae鱼类遗传距离最小,具有较近的亲缘关系。MSTN基因在卵形鲳鲹从受精卵到晶体出现期的13个阶段均表达量极低或不表达,组间差异不显著(P>0.05),而在初孵仔鱼期表达量迅速升高,极显著高于前13个时期(P<0.01)。MSTN基因可能在卵形鲳鲹孵化后发挥重要作用,而在孵化前作用微弱。
关键词(KeyWords): 卵形鲳鲹;MSTN;基因克隆;胚胎组织;生物信息学分析
基金项目(Foundation): 广西创新驱动发展专项资金项目(桂科AA17204080-3,桂科AA18242031-2);; 广西水产遗传育种与健康水产养殖重点实验室系统性课题(19-A-01-05)
作者(Author): 罗洪林;冯鹏霏;余艳玲;肖蕊;潘传燕;宋漫玲;张永德;
Email:
DOI: 10.16088/j.issn.1001-6600.2020042602
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