刘祎;叶雪梅;肖咪云;吕丽君;侯澄友;陆祖军;

• 1:广西师范大学生命科学学院
• 2:珍稀濒危动植物生态与环境保护教育部重点实验室
• 3:桂林润泰生物科技有限公司

摘要(Abstract)：

下箴刺桐碱是一种吲哚类生物碱,被认为是多种中草药的有效成分之一。彩色豆马勃Pisolithus tinctorius是一种硬皮马勃目的大型真菌,能在菌丝体内积累下箴刺桐碱。下箴刺桐碱具有比较强的天然荧光,但其荧光光谱与色氨酸几乎完全重叠,在用荧光方法筛选下箴刺桐碱高产菌株的过程中,共存的色氨酸会严重干扰下箴刺桐碱的定量。本研究构建一种色氨酸的快速荧光检测方法,运用此方法筛选出的一株彩色豆马勃菌株下箴刺桐碱含量(鲜质量含量)达到3.39nmol·mg^(-1)。

关键词(KeyWords)： 彩色豆马勃;筛选;下箴刺桐碱;色氨酸;荧光检测

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31060120);; 广西自然科学基金(13GXNSFAA019059);; 广西科技项目(桂科攻14124004-3-8,桂科攻14125006-81);; 广西大学生创新创业训练项目(201610602054)

作者(Authors): 刘祎;叶雪梅;肖咪云;吕丽君;侯澄友;陆祖军;

DOI: 10.16088/j.issn.1001-6600.2017.03.018

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